Product Description

* Description

Hs Prime Taq DNA Polymerase is designed for hot start PCR, a technique that enhances the specificity, sensitivity and yield of DNA amplification. In addition, the enzyme provides the convenience of reaction set-up at room temperature. the enzyme is inactive at room temperature, avoiding extension of non-specifically annealed promers or primer dimers and providing higher specificity of DNA amplification. The functional activity of the enzyme is restored during 10 minute incubation at 94ºC. the activated enzyme maintains the same functionality as Taq DNA polymerase : it catalyzes 5'→3' synthsis of DNA, has no detectable 3'→5' exonuclease activity.


* Model No.

Cat. No. G-7000▶ 250 Units X 1 - with 10 mM dNTPs Mixture
Cat. No. G-7001▶ 250 Units X 2 - with 10 mM dNTPs Mixture
Cat. No. G-7002▶ 250 Units X 4 - with 10 mM dNTPs Mixture 

▶ HSPrime Taq DNA Polymerase: 2.5 units/μl

▶ Storage buffer: 20 mM Tris-HCl (pH 8.0), 100 mM KCl, 0.5 mM EDTA, 0.1 mM DTT, 0.5% Tween20, 0.5% Nonidet P-40 and 50% Glycerol

▶ 10X reaction buffer: Tris-HCl (pH 9.0), PCR enhancers, (NH4)2SO4
and 20 mM MgCl2

▶ 10 mM dNTPs Mixture: 2.5 mM each of dATP, dCTP, dGTP and dTTP

▶ Use of monoclonal Taq Antibody
▶ Has the function of excellent Taq DNA Polymerase inhibition compared with polyclonal antibody and chemical forms
▶ Most suitable for a strict condition such as Multiplex PCR and Real Time PCR
▶ Possible to use smaller amount than general Taq DNA Polymerase
▶ HS Prime Taq DNA Polymerase can be used in T-vector cloning